qPCRas a primary screen in drug discovery

GauravJaggi, Frank Boeckler, Andreas Joerger and Alan Fersht, MRC -CPE

Date Posted: Monday, September 15, 2008

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We report the use of qPCR technique to follow the thermal unfolding of proteins by the binding of the dye SYPRO Orange, and exploit its potential as a robust and high-throughput primary screen for small molecule drug discovery.

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