Biphasic Reactions

Syrris Ltd

Date Posted: Monday, December 17, 2007

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A biphasic reaction involving electrophilic substitution of orcinol with a diazonium salt has been examined in the Africa flow system. The two-phase product solution was separated in the FLLEX module to give the azo product in the organic stream and an aqueous waste stream.

Orcinol (10-3 molar in ethyl acetate) and 4-nitrobenzenediazonium tetrafluoroborate (10-4 molar in water) were pumped at equal rates into a 250µl reaction chip held at 20°C with a reaction time of 1 minute. The resulting two-phase bright orange solution was separated in the FLLEX to yield the product azo compound in ethyl acetate and an aqueous waste stream.

The product azo solution was analysed by HPLC off-line and showed complete conversion of the starting diazonium salt.

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