Indirect UV Analysis of Amino Acids using by Capillary Zone Electrophoresis with LFII™ Detection


Date Posted: Monday, December 17, 2007

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A mixture of amino acids was successfully analysed using deltaDOT’s Peregrine HPCE at high pH to fully ionize each of the amino acid residues. Indirect UV detection was employed to visualise the 9 amino acids. Excellent resolution has been demonstrated between all 9 amino acid residues, with high reproducibility for migration time (<0.5% R.S.D.) and peak area (<2% R.S.D.). deltaDOT’s core technology of Label Free Intrinsic Imaging, (LFII™) may be applied to indirect UV measurements for the analysis of substances including carbohydrates, lipids and sugars, which typically absorb little in the 200-300nm spectral range.

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