Automated Nanolitre Hit Automated Nanolitre Hit - Picking using a Mosquito® X1 Low Volume Pipettor
Joby Jenkins, Rob Lewis, Tristan Cope, Wayne Bowen,
TTP LabtechDate Posted: Thursday, March 02, 2006
A prerequisite for efficient primary screening is the automated selection of “hits” for confirmation and secondary profiling. Solutions exist for low density microplates, however, the 1536 well format presents significant challenges for many liquid handling systems. The mosquito® X1 offers precision sampling of any individual well in 48-, 96-, 384- or 1536-well plates. This enables researchers to quickly select small volumes of “hits” from primary screening plates and transfer them directly to the next screening stage without further dilution. mosquito® X1’s disposable pipette tips guarantee zero cross-contamination.
The system’s unique positive displacement pipetting technology allows it to pipette accurately and reproducibly throughout the 25 nL - 1.2 µL range, producing CVs of <10% at 25 nL with a cycle time of around 7 seconds. The mosquito X1 can be integrated with common plate handlers for vastly increased walk-away time.
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