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Production of prostanoids in the plant Arabidopsis thaliana

Maged E. Mohamed and Colin M. Lazarus., School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, United Kingdom.

Date Posted: Thursday, October 02, 2008

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Prostanoids originate from PGHS action on the 20-carbon polyunsaturated fatty acid: DGLA, AA and EPA. We used cDNA expression in Saccharomyces cerevisiae and enzyme immunoassays to compare the activities of two mouse PGHS isoforms and cDNAs encoding mouse prostaglandin I synthase and thromboxane A synthase and genomic DNA encoding Trypanosoma brucei prostaglandin F synthase. All genes were active and were put in Transgenic Arabidopsis thaliana plants producing DGLA.

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