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Analysis of E.coli lysate using Peregrine High Performance Capillary Electrophoresis (HPCE) with Label Free Intrinsic Imaging (LFII™)


Date Posted: Monday, December 17, 2007

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Analytical instruments with the capability of analyzing complex mixture of bio-molecules with high precision, excellent reproducibility and quantitative indications are highly sought after in fields ranging from routine quality control procedure in bio-molecule production to biomarker discovery and diagnostic industry. This application note describes the high resolution separation and subsequent molecular weight characterization of four of the most abundant protein in E. coli cell lysate, using deltaDOT’s Peregrine HPCE system and proprietary LFII™ technology. Its capability to produce high quality reproducible data in terms of peak mobility (<1% RSD) and peak area (<5% RSD) was also demonstrated.

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