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Porvair Sciences Releases Application Note to Describe an Automated SPE Method

Date Posted: Tuesday, March 20, 2007

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Porvair Sciences Ltd has released an application note that describes an automated SPE method for preparing samples for a rapid assay of an anxiolytic compound in human plasma, which employs liquid chromatography with tandem mass spectrometric (LC/MS/MS) detection.

Traditional methods employing Solid Phase Extraction (SPE) and LC/MS/MS were found not to have the throughput required to match the capacity of the assay that was being used to support elucidation of pharmacokinetic parameters of the target compound in Phase 1 trials.

The SPE method was therefore adapted to employ the Microlute™ - a high performance 96-well SPE microplate system. The application note describes how the Microlute™ system can enable a batch of samples to be prepared for injection in less than half the time that is required to analyse them.

The developed method using the Microlute™ was validated over a concentration range of 100 to 10,000 pg /ml. Recovery of the SPE method was approximately 60%, and consistent across the calibration range. The authors describe how the Microlute™ SPE method is free from LC/MS matrix effects that may influence the ionisation of the compound(s) of interest.

According to Porvair Sciences, the Microlute™ is designed to offers all the advantages of automated and high throughput SPE sample preparation in a microplate format capable of processing 96 samples in one go repeatedly.

Each well on the Microlute™ has an individua drain spout ensuring 100% sample transfer and zero crossover contamination. Working closely with the automated liquid handling companies has ensured that the Microlute™ delivers high productivity, trouble-free operation with all robotic sample handling and preparation systems.

Further Information: http://www.porvair-sciences.com

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