1.
Introduction: Why Proteomics?
Gene expression information is often
insufficient to determine the ultimate cause of a condition or disease.
2.
Scientific Background: The Challenge of Greater Complexity
The differences between proteomics and
genomics far outweigh the similarities, but the two fields can be highly
complementary.
2.1 Comparing Proteomics with Genomics
Similarities
Differences
2.2 Using Proteomics and Genomics in a Complementary Fashion
3. The
Current and Emerging Technologies: The Search for High Throughput
The major goal for proteomics leaders is to
accelerate high-throughput capacity, but as the field enters the next level of
analysis—further elucidation of protein function—there will be an increasing
need for better systems of data capture and analysis, as well as models capable
of representing complex networks.
3.1 The First Level of Analysis: Protein Separation, Identification, and Characterization
Sample Preparation
2-D Gel
Electrophoresis
Staining
Image Analysis
Mass Spectrometry
Protein Chips
Databases
Bioinformatics
3.2 The Next Level of Analysis: Tools And Approaches for Protein Function Studies
Quantification
High-Throughput Expression
Protein-Protein Interactions
Protein Function
What’s Next?
4.
Business and Strategic Considerations: The Leaders Are Far Ahead
A handful of companies have taken the lead in
this field’s various markets—for services, specialized products, and
supplies—and despite the growing demand in these markets, proteomics newcomers
face a challenge in catching the leaders.
4.1 Industry Leaders
4.2 Selected Tool and Service Providers
4.3 Bench-Top Platform Technology Providers
5.
Applications: Early Signs of Valuable Potential
Proteomics is already showing substantial
promise in key areas of drug development—
toxicology and the identification of surrogate
markers of drug response—as well as in studies of pathogens and other primitive
organisms.
5.1 Basic Research
5.2 Diagnostics and Prognostic Indicators
5.3 Drug Discovery and Development
Target Identification snd
Validation
Pathway Elucidation
Mode of Action
Toxicology
Surrogate Markers of Drug Response
Drug Resistance
5.4 Pathogen Studies
5.5 Agriculture and Other Areas
6.
Outlook: New Tools and a Context for Managing Data
As genomic sequence information becomes a
commodity, a new set of criteria— expertise in bioinformatics, protein
function, and modeling complex systems— will determine this field’s leaders.
6.1 Key Remaining Issues
Is 2-D Gel Electrophoresis Replaceable?
Access to Genetic Sequence
Information
Plain Skepticism
6.2 The Future of Proteomics
6.3 Commentaries from Editorial Board Members
Appendix
A: Table of Companies with Proteomics Related Activities
Appendix B: Company Profiles