Microarrays
and Related Technologies:
Miniaturization
and Acceleration of Genomics Research
INTRODUCTION: Why DNA Arrays?
Chapter 2
SCIENTIFIC OVERVIEW: Major Types of DNA Array Applications and Forces
Driving the Market
2.1 DNA Array Applications
Single Nucleotide Polymorphism Studies
Studies of Gene Expression Patterns/Gene Function
2.2 Factors Driving the Development of DNA Arrays
Chapter 3
CURRENT AND EMERGING TECHNOLOGIES: Specific Array Types and Related
Technologies
3.1 DNA Hybridization Array Types
Single Nucleotide Polymorphism Studies
Microarrays
Sidebar: The
Stokes Shift
Oligonucleotide Arrays/GeneChips
Macroarrays
Microelectronic Arrays
Accuracy Issues
3.2 Bead-Based Arrays
3.3 Gel-Pad Arrays
3.4 Protein Arrays
Protein Chip Types/Challenges
Recent Advances Sidebar: An Interview with Gavin MacBeath of the Harvard Center
for Genomics Research
3.5 Small-Scale, Microfluidics-Based Approaches for Genomic Analysis
Caliper Technologies
Orchid BioSciences
Outlook
Chapter 4
CREATING, READING, AND ANALYZING ARRAYS: Major Tools and Technologies
4.1 Creating Arrays
Direct Deposition
Indirect Deposition/Ink-Jet-Piezoelectric
Photolithography
Substrates
4.2 Labeling
Target Amplification
Signal Amplification
4.3 Imagers for Detection
Fluorescence Imagers
Phosphorimagers
4.4 Spectrometry
4.5 Software/Bioinformatics
Image Analysis
Data Analysis
Data-Integration/Relational Databases
Challenges
Chapter 5
THE BUSINESS OF DNA ARRAYS: Market Forecasts, Dealmaking Trends, and
Industry Outlook
5.1 Market Forecast
Overall Market Forecast, 2000-2006: Robust Growth Expected
Market Shares by Company, 2000-2006: Affymetrix to Remain the Leader
Sidebar: Array
Field Challenged by Patent Litigation, Yet Opportunities Remain
5.2 Dealmaking in the Array Field
Top Dealmakers and Their Deals
Dealmaking in Protein Arrays
5.3 Outlook for the Field and Remaining Challenges
Outlook: High Cost of Existing Technologies, the Need for Continuing
Improvements in Bioinformatics Tools and Data, Regulatory Hurdles for
Microarrays as Diagnostics